Elucidating Mechanisms of Fine Genetic Control by a sRNA in Pathogenic Bacterium Salmonella typhimurium

Elton Chan : Molecular and Cell Biology/Microbial Biology

Mentor: Professor Fenyong Liu

The bacterium Salmonella is a significant cause of food-borne disease. Its pathogenesis depends on the type III secretion systems (T3SSs) that were acquired by horizontal gene transfer; the invasion of Salmonella into the host cells requires appropriate expression of T3SSs. Recent research has identified small non-coding RNAs (sRNAs) as a class of regulators that fine tune gene expression required for bacterial physiology and pathogenesis. Elton will investigate the specific interaction between one of these newly discovered Salmonella sRNA and its predicted candidate targets; he will characterize the interaction between IsrM and its cognate targets, HilE and SopA, and identify the mechanisms of these interactions. The research work on the interaction between this sRNA and its targets will contribute towards a more complete understanding of the molecular coordination of Salmonella pathogenesis.